Evaluation of salivary α-L-Fucose and its related parameters in periodontitis

  • Mohammed Ahmed Wsoo Department of Chemistry, faculty of Education, University of Raparin, Rania/ Sulaimani/Iraq.
  • Bakhtiar Mohiadeen Ahmed Department of Basic Science, College of Dentistry, Hawler Medical University, Erbil, Iraq.
Keywords: Periodontitis, salivary fucose, salivary minerals, glycoconjugates, glycoproteins

Abstract

Background and objective: Periodontitis is one of the most widespread oral diseases in Kurdistan. Saliva can be used as a noninvasive diagnostic fluid to measure chemical biomarkers released during oral disease, so this work is directed to focus on the study of salivary fucose (a glycoprotein component) and its related parameters in periodontitis, to assess the possibility of using them as indicators for the disease and its progress.

Methods: The present work included 79 individuals. They were grouped into healthy (32), advanced periodontitis (20) and moderate periodontitis(27) subjects. Their age was ranged between (20-60) years. Unstimulated whole saliva samples were collected from all the groups. The samples were used for the estimation of salivary; total fucose (TF), protein bound fucose (PBF), protein bound hexose (PBH), lipid associated fucose (LAF), total protein (TP), total calcium (TCa), zinc (Zn), and salivary magnesium (Mg).

Results: Salivary TF, LAF, FF, PBF, TP and TCa were significantly increased in both advanced and moderate periodontic groups comparing to healthy individuals, while there were no significant differences in salivary PBH, Mg and Mg/Ca levels in periodontic groups when compared to normal. Salivary TF/TP, PBF/TP, PBH/TP and FF/TP ratios and salivary zinc were significantly reduced in periodontic males.

Conclusion: On the basis of the above results, it can be concluded that these biochemical parameters, may be used as indicators for the extent of periodontal tissue damage, thus they can be used in the identification of periodontitis progresses and treatment follow up.

References

Kim, S-C.; Kim, O-S.; Kim, O-J.; Kim, Y-J.; Chung, H-J. Antioxidant profile of whole saliva after scaling and root planing in periodontal disease. Journal of Periodontal & Implant Science. 2010;40:164-71.

Dhotre, P.S.; Suryakar, A.N.; Bhogade R.B. Oxidative stress in periodontitis: A critical link to cardovascular disease. Biomedical Res. 2011; 22(2):178-82.

Gieorgijewska, A.; Adamczyk-Mościcka, M.; Pawlik, M.; Krajewski, J.; Górska, R.; Lank O.; et al. Periodontal status evaluation in patients with diagnosed IBD: Crohn’s disease and ulcerative colitis. Gastroenterologia Polska. 2011;18 (1): 8-11.

De Almeida, P.D-V.; Grégio, A.M.T.; Machado, M.A.N.; De Lima, A.A.S.; Azevedo, L.R. Saliva composition and functions: A comprehensive review. Journal Contemporary Dental Practice. 2008;9(3): 1-11.

Preethi, B.P.; Reshma, D.; Anand, P. Evaluation of flow rate, pH, buffering capacity, calcium, total proteins and total antioxidant capacity levels of saliva in caries free and caries active children: An in vivo study. Ind. J. Clin. Biochem. 2010;25(4): 425-8.

Miller, C.S.; King, J.C.P.; Langub, M.C.; Kryscio, R.J.; Thomas, M.V. Salivary biomarkers of existing periodontal Disease. American Dental Association. 2006;137: 322-29.

Tomita, Y.; Nahoko, M.; Sumie, Y. Lipid in human parotid saliva with regard to caries experience. Journal of Oleo Science. 2008;57(2): 115-21.

Bimstein, E.; Small, P.A.; Magnusson, I. Leukocyte Esterase and protein levels in saliva, as indicators of gingival and periodontal diseases in children. Pediatric Dentistry. 2004;26(4): 310-5.

Kamerling, J. Structural studies on glycoprotein glycans. Pure & Appl. Chern. 1994;66(10/11): 2235-8.

Haddad, A. Radioactive fucose as a tool for studying glycoprotein secretion. Brazilian journal of medical and biological research. 1998;13(2):207-13.

Joseph, A. Serum and Salivary Sialic acid levels in different grades of oral squamous cell carcinoma and premalignancy comparative and correlative study. MSc. Thesis. Rajiv Gandhi University of Health Sciences, Bangalore, 2006.

Becker, D.J.; Lowe, J B. Fucose : biosynthesis and biological function in mammals. Glycobiology. 2003;13(7): 41R-53R.

Abbass, L.B; Ahmed, S.A. Serum total α-L-fucose and related parameters in breast cancer as tumor marker. Journal of family Medicine.2011; 9(1): 3-8.

Park, D.; Ryu, K.S.; Choi ,J.K.; Park, C. Characterization and role of fucose mutarotase in mammalian cells. Glycobiology. 2007;17(9): 955-62.

Shetty, P. K.; Pattabiraman T.N. Salivary glycoproteins as indicators of oral diseases. Indian J. Clin. Biochem.2004;19(1): 97-101.

Shah, M.; Telang, S.; Raval, G.; Shah, P.; Patel, P.S. Serum fucosylation changes in oral cancer and oral precancerous condition: Alpha-L-fucosidase as a marker. J. Cancer. 2008;113(2):336-46

Mohammed, S. A. Periodontal health status among young adult patients aged (20-29) years attending periodontology teaching clinic (in Hawler city). MSc. thesis, College of Dentistry, Hawler medical Univeristy,2009.

Fábián, T.K.; Fejerdy, P.; Csermely, P. Saliva in health and disease, Chemical Biology of. Wiley encyclopedia of chemical biology. 2007;1-9.

Gornall, A. G.; Bardawill, C. J. and David, M. M. Determination of serum proteins by means of the biuret reaction. J. Biol. Chem. 1949;77: 751-66.

Tietz N.W. Text book of clinical chemistry, 3rd Ed. C.A. Curtis, E.R. Silverman L.M., Christensen R.H. 1995; P.523-4.

Dische, Z. and Shettles, L. B. 1948 Specific colors reactions of methyl pentoses and spectrophotometric micromethod for their determination J. Biol. Chem. 1948;175: 595-604.

Abbas, L.B. Changes in α-L-fucose and related parameters levels in Leukemia, Ph.D thesis, University of Salahaddin, College of Medincine-Erbil, 2004.

Yousif, N.,F. (2005) α-L-fucose as a possible diagnostic marker with other related parameters in thyroid dysfunction patients, Ph.D thesis, College of Ibn Al-Haitham, University of Baghdad, Baghdad, 2005.

Manoharan, S.; Padmanabhan, M.; Kolanjiappan, K.; Ramachandran, C. R. and Suresh, K. Analysis of glycoconjugates in patients with oral squamous cell carcinoma. Clinica Chimica Acta. 2004; 339: 91-6.

Zangana, Z. A. A. α-L-Fucose among other biochemical parameters as a possible diagnostic parameter in Thalssemic patients. PhD Thesis, College of Education, Ibn al-haitham, University of Baghdad, 2006.

Novaes, A.B.; Shapiro, L.; Fillios, LC.; Wood, N. Gingival fluid fucose to protein ratios as indicators of the severity of periodontal disease. J Periodontol. 1980;51(2):88-94.

Koss, M.A.; Castro, C.E.; Salum, K.M.; Lopez, M.E. Changes in saliva protein composition in patients with periodontal disease. Acta Odontol Latinoam. 2009;22(2):105-112

Henskens, Y.M.; van der Velden, U.; Veerman, E.C.; Nieuw Amerongen, A.V. Protein, albumin and cystatin concentrations in saliva of healthy subjects and of patients with gingivitis or periodontitis. J Periodontal Res. 1993;28: 43-8.

Nieminen, A.; Norduland, L.; Uitto, V.J. The effect of treatment on the periodontitis. J. periodontal.1993; 64(4):297-301

Kiss, E. Salivary Electrolytes, Focused on Salivary Calcium Level and the Periodontal State in Healthy Smoking and Non-Smoking Women, PhD. Thesis, Department of Periodontology, Faculty of Dentistry, University of Szeged. 2010;PP:1-14

Cochran, D.L. Inflammation and Bone Loss in Periodontal Disease. J Periodontol. 2008;79(8):1569-75.

Kuraner, T.; Beksac, M.S.; Kayakirilmaz, K.; Cağlayan, F.; Onderoğlu, L.S.; Ozgünes, H . Serum and parotid saliva testosterone, calcium, magnesium, and zinc levels in males, with and without periodontitis. Biol Trace Elem Res. 1991;31(1):43-9.

Ma, Z.J.; Zhang, J.Z. Changes in serum zinc level of periodontitis with kidney deficiency. Zhongguo Zhong Xi Yi Jie He Za Zhi. 1993;13(10):606-7.

Martin, R.R.; Naftel, S.J.; Nelson, A.J.; Edwards, M.; Mithoowani, H.; Stakiw, J. Synchrotron radiation analysis of possible correlations between metal status in human cementum and periodontal disease. J Synchrotron Radiat.2010;17(2): 263-7.

Yoshihara, A.; Iwasaki, M.; Miyazaki, H. Mineral content of calcium and magnesium in the serum and longitudinal periodontal progression in Japanese elderly smokers. J Clin Periodontol.2011;38(11): 992-7.

Published
2018-10-08
How to Cite
Wsoo, M., & Ahmed, B. (2018). Evaluation of salivary α-L-Fucose and its related parameters in periodontitis. Zanco Journal of Medical Sciences (Zanco J Med Sci), 17(3), 563 - 569. https://doi.org/10.15218/zjms.2013.0049
Section
Original Articles